Before I could begin culturing, I needed to build out a sterile lab space to work in. There are a couple of primary pieces of equipment that I will be relying on. The first, is a sterilizer. I'll be working with a 25 Quart steam sterilizer from All-American. This will allow me to sterilize my tools, and smaller volume substrates. The second, is a laminar flow hood. A flow hood is essentially a space which can be thoroughly cleaned and sterilized, and will blow clean air over the work space, creating a *mostly bacteria/contaminate free zone. Rather than purchasing a pre-made flow hood, I chose to build my own. A primary sketch below shows the main component, a HEPA filter, with a contained box at the back, and a plastic hood at the front. Onto the wooden box, is fitted a blower, rated to the CFM (cubic feet/minute) that this particular HEPA filter requires.